Isocitrate Dehydrogenase anticorps (Biotin)

N° du produit ABIN568271

L’anticorps Lapin Polyclonal anti-Isocitrate Dehydrogenase a été validé pour WB, IF, IP, EIA, DB, ID et RIA. Il convient pour détecter Isocitrate Dehydrogenase dans des échantillons de Porc.

Aperçu rapide pour Isocitrate Dehydrogenase anticorps (Biotin) (ABIN568271)

Antigène: Isocitrate Dehydrogenase (IDH)

Reactivité: Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Isocitrate Dehydrogenase est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Détail du produit anti-Isocitrate Dehydrogenase anticorps (Biotin)

Attributs du produit: Molar Ratio: Biotin/IgG ~6.5

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogène: Isocitrate Dehydrogenase isolated and purified from Porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore by adding 1.0 mL sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Détails sur Isocitrate Dehydrogenase

Antigène: Isocitrate Dehydrogenase (IDH)

Autre désignation: Isocitrate Dehydrogenase / IDH

Sujet: Isocitrate dehydrogenase is an enzyme involved in the citric acid cycle. It is 416 amino acids long with a molecular weight of approximately 45 kDa. Isocitrate dehydrogenase enzymes catalyze the oxidative decarboxylation of isocitrate to produce alfa ketoglutarate. The human genome has 5 IDH genes coding for 3 IDH enzymes. The IDH1 and IDH2 require nicotinamide adeninedinucleotide phosphate (NADP) as co-substrate, whereas IDH3 require nicotinamide adenine dinucleotide (NAD). The IDH2 and 3 are localized in mitochondria and are actively involved in the citric acid cycle (TCA) for energy production in contrast, IDH1 is localized in cytoplasm and peroxisomes where it generates NADPH, reduced form of NADP for biosynthetic and other types of reaction. Since alfa KG and NADPH both are intermediately substrate for a number of cellular process, which allows the possibility of oncogenic or tumor suppressive activities of IDH1.Synonyms: Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD, cytoplasmic Isocitrate dehydrogenase [NADP]

ID gène: 9823

UniProt: P20304