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Luciferase anticorps (Biotin)

Cet anticorps anti-Luciferase est un anticorps Lapin Polyclonal détectant Luciferase dans WB, IF, IP, DB, EIA, RIA et ID. Adapté pour Photinus pyralis.
N° du produit ABIN568305

Aperçu rapide pour Luciferase anticorps (Biotin) (ABIN568305)

Antigène

Voir toutes Luciferase Anticorps
Luciferase

Reactivité

  • 31
  • 19
  • 8
  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
Photinus pyralis

Hôte

  • 45
  • 14
  • 10
  • 1
  • 1
Lapin

Clonalité

  • 43
  • 28
Polyclonal

Conjugué

  • 31
  • 9
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Luciferase est conjugé à/à la Biotin

Application

  • 39
  • 25
  • 13
  • 13
  • 10
  • 8
  • 8
  • 8
  • 8
  • 7
  • 6
  • 4
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Dot Blot (DB), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID)
  • Attributs du produit

    Molar Ratio: Biotin/IgG: ~1.7

    Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

    Immunogène

    Luciferase isolated and purified from Photinus pyralis. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    Restore by adding 1.0 mL of sterile distilled water.

    Concentration

    10 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins.

    Agent conservateur

    Without preservative

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Antigène

    Luciferase

    Sujet

    Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.Synonyms: Luciferin 4-monooxygenase

    ID gène

    7054

    UniProt

    P08659
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