Pepsin anticorps

N° du produit ABIN568351

Cet anticorps Lapin Polyclonal détecte spécifiquement Pepsin dans WB, IP, EIA, IF, ID, DB et RIA. Il présente une réactivité envers Porc.

Aperçu rapide pour Pepsin anticorps (ABIN568351)

Antigène: Pepsin

Reactivité: Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Pepsin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunodiffusion (ID), Dot Blot (DB), Radioimmunoassay (RIA)

Détail du produit anti-Pepsin anticorps

Specificité: Pepsin from Porcine stomach mucosa. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogène: Pepsin isolated and purified from Porcine stomach mucosa. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 1.0 mL sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Détails sur Pepsin

Antigène: Pepsin

Sujet: Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.Synonyms: PGA, PGA3, PGA4, PGA5, Pepsin A, Pepsinogen A, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I

ID gène: 396892

NCBI Accession: NP_999038

UniProt: P00791