Cet anticorps Lapin Polyclonal détecte spécifiquement Glucose-6-Phosphate Isomerase (PGI1) dans DB, EIA, ID, IF, IP, RIA et WB. Il présente une réactivité avec des échantillons de Saccharomyces cerevisiae. Il a été cité dans 4+ publications.
Aperçu rapide pour Glucose-6-Phosphate Isomerase (PGI1) anticorps (ABIN568369)
Antigène
Glucose-6-Phosphate Isomerase (PGI1)
Reactivité
Saccharomyces cerevisiae
Hôte
Lapin
Clonalité
Polyclonal
Conjugué
Inconjugué
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
Specificité
IgG fraction of polyclonal rabbit antiserum to Phosphoglucose Isomerase from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogène
Phosphoglucose isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10,0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.
Cáceres-Gorriti, Carmona, Barrès, Rahimi, Létourneau, Tonin, Provencher, Mes-Masson: "RAN nucleo-cytoplasmic transport and mitotic spindle assembly partners XPO7 and TPX2 are new prognostic biomarkers in serous epithelial ovarian cancer." dans: PLoS ONE, Vol. 9, Issue 3, pp. e91000, (2016) (PubMed).
Miura, Ueda: "Evaluation of unconventional protein secretion in Saccharomyces cerevisiae." dans: Methods in molecular biology (Clifton, N.J.), Vol. 1270, pp. 51-70, (2015) (PubMed).
Miettinen, Björklund: "Modified ribosome profiling reveals high abundance of ribosome protected mRNA fragments derived from 3' untranslated regions." dans: Nucleic acids research, Vol. 43, Issue 2, pp. 1019-34, (2015) (PubMed).
Ryu, Kwak, Lee, Kang, Oh, Lee, Kim, Ma, Kim: "Sulfur amino acid metabolism in doxorubicin-resistant breast cancer cells." dans: Toxicology and applied pharmacology, Vol. 255, Issue 1, pp. 94-102, (2011) (PubMed).