CCL7 anticorps
Aperçu rapide pour CCL7 anticorps (ABIN5687634)
Antigène
Voir toutes CCL7 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Anti-mMCP-3 specific antibody was purified by affinity chromatography employing immobilized mMCP-3 matrix.
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Immunogène
- Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mMCP-3 (murine Macrophage/Monocyte chemotactic protein-3).
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Indications d'application
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Murine MCP-3 (100.0 ng/mL), a concentration of 4.0 - 6.0 μ,g/mL of this antibody is required..
ELISA:
To detect mMCP-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-3.
Sandwich:
To detect mMCP-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MCP-3 (XP-5223Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
Western Blot:
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Stock
- -20 °C
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Stockage commentaire
- MCP-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))
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Autre désignation
- MCP-3
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ID gène
- 20306
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UniProt
- Q03366
Antigène
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