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IL-3 beta anticorps

L’anticorps Chèvre Polyclonal anti-IL-3 beta a été validé pour WB, ELISA et Neut. Il convient pour détecter IL-3 beta dans des échantillons de Rat.
N° du produit ABIN5687647

Aperçu rapide pour IL-3 beta anticorps (ABIN5687647)

Antigène

IL-3 beta (Interleukin 3 beta (IL-3 beta))

Reactivité

Rat

Hôte

  • 4
Chèvre

Clonalité

  • 4
Polyclonal

Conjugué

  • 2
  • 1
  • 1
Cet anticorp IL-3 beta est non-conjugé

Application

  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Neutralization (Neut)
  • Purification

    Anti-rat IL-3β, specific antibody was purified by affinity chromatography employing immobilized hIL-3β, matrix.

    Immunogène

    Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-3β, (rat IL-3 beta).
  • Indications d'application

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-3β, (150 ng/mL), a concentration of 0.68 - 1.03 μ,g/mL of this antibody is required.

    ELISA:

    To detect Rat IL-3β, by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3β,..

    Sandwich:

    To detect Rat IL-3β, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-3β, (XP-5187Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3β,.

    Western Blot:

    To detect Rat IL-3β, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3β, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Stock

    -20 °C

    Stockage commentaire

    IL-3B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • Antigène

    IL-3 beta (Interleukin 3 beta (IL-3 beta))

    Autre désignation

    IL-3B

    Sujet

    IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells

    ID gène

    24495

    UniProt

    P97688
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