CCL8 anticorps
Aperçu rapide pour CCL8 anticorps (ABIN5688696)
Antigène
Voir toutes CCL8 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Purification
- The monoclonal antibody against human MCP-2 designated as VL-1 was purified from ascites fluid on a Protein A affinity column.
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Immunogène
- The monoclonal antibody was derived form BALB/c X ICR F1 mice and belongs to the IgG1K isotype.
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Indications d'application
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100 ng/mL), a concentration of 0.8-1.0 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 1.0-2.0 μ,g/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-2 when used with our biotinylated antigen affinity purified anti-human MCP-2 (XP-5224Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.
Western Blot:
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- MCP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
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- CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))
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Autre désignation
- MCP-2
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Sujet
- MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.
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ID gène
- 6355
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UniProt
- P80075
Antigène
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