CCL7 anticorps

N° du produit ABIN5688702

Cet anticorps Souris Monoclonal détecte spécifiquement CCL7 dans WB, ELISA et Neut. Il présente une réactivité envers Humain.

Aperçu rapide pour CCL7 anticorps (ABIN5688702)

Antigène: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CCL7 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: PEPY042_hMCP3

Détail du produit anti-CCL7 anticorps

Purification: This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.

Immunogène: The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMCP-3 as the immunizing antigen.

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-3 (100 ng/mL), a concentration of 1 - 3 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect recombinant human MCP-3 when used with our biotinylated antigen affinity purified anti-human MCP-3 (XP-5221Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:

To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: 4 °C,-20 °C

Stockage commentaire: MCP-3 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Détails sur CCL7

Antigène: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Autre désignation: MCP-3

Sujet: MCP-3 is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

ID gène: 6354

UniProt: P80098