MARCKS anticorps (pSer152, pSer156)

N° du produit ABIN5688889

L’anticorps anti-MARCKS Polyclonal Lapin est utilisé pour la détection de MARCKS dans des échantillons de Humain, Souris, Rat et Xenopus laevis. Il a été validé pour WB.

Aperçu rapide pour MARCKS anticorps (pSer152, pSer156) (ABIN5688889)

Antigène: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivité: Humain, Souris, Rat, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MARCKS est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-MARCKS anticorps

Épitope: pSer152, pSer156

Specificité: MARCKS antibody is specific for MARCKS phosphorylated at Ser152 and Ser156.

Purification: Affinity Purified

Immunogène: MARCKS (Ser152/156) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152 and phospho-Ser156 of rat MARCKS.

Information d'application

Indications d'application: Immunolabeling of the 87  kDa band in rat brain homogenate is blocked by the Ser152/156 phosphopeptide but not by the non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) not yet determined. Human, mouse, rat, and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Stock: -20 °C

Stockage commentaire: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Détails sur MARCKS

Antigène: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Autre désignation: MARCKS

Sujet: Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Moreover, MARCKS is a member of a family of calmodulin binding proteins. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.

Poids moléculaire: 87 kDa

ID gène: 25603

UniProt: P30009