CCL4 anticorps
Aperçu rapide pour CCL4 anticorps (ABIN5689081)
Antigène
Voir toutes CCL4 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Anti-hMIP-1-beta specific antibody was purified by affinity chromatography employing immobilized hMIP-1-beta matrix.
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Immunogène
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1-beta.
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Indications d'application
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1-beta (50.00 ng/mL), a concentration of 1.75 - 3.0 μ,g/mL of this antibody is required.
ELISA:
To detect hMIP-1-beta by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-1-beta.
Sandwich:
To detect hMIP-1β, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-1β, (XP-5235Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1β,.
Western Blot:
To detect hMIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Stock
- -20 °C
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Stockage commentaire
- MIP-1B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
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Autre désignation
- MIP-1B
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ID gène
- 6351
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UniProt
- P13236
Antigène
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