CCL3 anticorps

N° du produit ABIN5689165

L’anticorps Lapin Polyclonal anti-CCL3 a été validé pour WB, ELISA et Neut. Il convient pour détecter CCL3 dans des échantillons de Souris.

Aperçu rapide pour CCL3 anticorps (ABIN5689165)

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL3 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-CCL3 anticorps

Purification: Anti-mMIP-1-alpha specific antibody was purified by affinity chromatography employing immobilized mMIP-1-alpha matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1-alpha (murine Macrophage Inflammatory Protein-1-alpha).

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-1-alpha (10 ng/mL), a concentration of 2.0 - 5.0 μ,g/mL of this antibody is required.

ELISA:

To detect mMIP-1-alpha by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-1-alpha.

Sandwich:

To detect hMIP-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MIP-1α, (XP-5234Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1α,.

Western Blot:

To detect mMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: MIP-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur CCL3

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Autre désignation: MIP-1a

Sujet: MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

ID gène: 20302

UniProt: P10855

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy