VEGF anticorps

N° du produit ABIN5689180

L’anticorps Lapin Polyclonal anti-VEGF a été validé pour WB, ELISA et Neut. Il convient pour détecter VEGF dans des échantillons de Souris.

Aperçu rapide pour VEGF anticorps (ABIN5689180)

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGF est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-VEGF anticorps

Purification: Anti-mVEGF specific antibody was purified by affinity chromatography employing immobilized mVEGF matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF (murine VEGF).

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (50.00 ng/mL), a concentration of 1.0 2.0 μ,g/mL of this antibody is required.

ELISA:

To detect mVEGF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mVEGF.

Sandwich:

To detect mVEGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine VEGF (XP-5293Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF.

Western Blot:
To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: VEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur VEGF

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Autre désignation: VEGF

ID gène: 22339