IL-10 anticorps

N° du produit ABIN5689203

Cet anticorps Lapin Polyclonal détecte spécifiquement IL-10 dans WB, ELISA et Neut. Il présente une réactivité envers Rat.

Aperçu rapide pour IL-10 anticorps (ABIN5689203)

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-10 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-IL-10 anticorps

Purification: Anti-rIL-10 specific antibody was purified by affinity chromatography employing immobilized rIL-10 matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rIL-10 (rat Interleukin-10).

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of rIL-10 (30.00 ng/mL), a concentration of 0.45 0.8 mg/mL of this antibody is required.

ELISA:

To detect rIL-10 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rIL-10.

Sandwich

To detect Rat IL-10 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-10 (XP-5163Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.

Western Blot:

To detect rIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: IL-10 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur IL-10

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Autre désignation: IL-10

Sujet: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL.

ID gène: 25325

UniProt: P29456

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints