IL-33 anticorps

N° du produit ABIN5690010

Cet anticorps Lapin Polyclonal détecte spécifiquement IL-33 dans WB, ELISA, IHC et Neut. Il présente une réactivité envers Humain.

Aperçu rapide pour IL-33 anticorps (ABIN5690010)

Antigène: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-33 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut)

Détail du produit anti-IL-33 anticorps

Purification: IL-33 specific antibody was purified by affinity chromatography employing immobilized hIL-33 matrix.

Immunogène: E.coli derived Recombinant Human IL-33

Information d'application

Indications d'application: ELISA:
Sandwich:
To detect hIL-33 by sandwich ELISA (using 100μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Polyclonal Biotinylated Anti-Human IL-33 (38-193) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-33.

Western Blot:

To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/mL), a concentration of 0.2-0.4 μ,g/mL of this antibody is required.

Immunohistochemistry:

This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 mg/mL-0.25 mg/mL with an overnight incubation at 4˚,C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6.0 sodium citrate buffer is recommended.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur IL-33

Antigène: IL-33 (IL33) (Interleukin 33 (IL33))

Autre désignation: IL-33

ID gène: 90865

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response