VEGF anticorps (Biotin)

N° du produit ABIN5690497

L’anticorps Lapin Polyclonal anti-VEGF a été validé pour WB et ELISA. Il convient pour détecter VEGF dans des échantillons de Rat.

Aperçu rapide pour VEGF anticorps (Biotin) (ABIN5690497)

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGF est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-VEGF anticorps (Biotin)

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat VEGF (Rat Vascular Endothelial Growth Factor). Rat VEGF specific antibody was purified by affinity chromatography and then biotinylated.

Information d'application

Indications d'application: ELISA:
Direct:
To detect Rat VEGF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.

Sandwich
To detect Rat VEGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Rat VEGF as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.

Western Blot:
To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: VEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur VEGF

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Autre désignation: VEGF

ID gène: 83785

UniProt: P16612