CCL4 anticorps
Aperçu rapide pour CCL4 anticorps (ABIN5690510)
Antigène
Voir toutes CCL4 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
-
-
Immunogène
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1β,. Murine MIP-1β, specific antibody was purified by affinity chromatography employing immobilized mMIP-1β, matrix.
-
-
-
-
Indications d'application
-
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Murine MIP-1β, (100.0 ng/mL), a concentration of 8.0-10.0 μ,g/mL of this antibody is required.
ELISA:
Indirect:
To detect mMIP-1b by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.
Sandwich
To detect mMIP-1b by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MIP-1b as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.
Western Blot:
To detect mMIP-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Stock
- -20 °C
-
Stockage commentaire
- MIP-1B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
-
-
- CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
-
Autre désignation
- MIP-1B
-
ID gène
- 20303
-
UniProt
- P14097
Antigène
-