Defensin beta 4 anticorps (Biotin)
Aperçu rapide pour Defensin beta 4 anticorps (Biotin) (ABIN5690601)
Antigène
Voir toutes Defensin beta 4 (DEFB4) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Immunogène
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-4 (Human Beta Defensin-4). hBD-4 specific antibody was purified by affinity chromatography and then biotinylated.
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Indications d'application
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ELISA:
Direct:
To detect hBD-4 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-4.
Sandwich
To detect hBD-4 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human BD-4 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-4.
Western Blot:
To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Stock
- -20 °C
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Stockage commentaire
- BD-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Defensin beta 4 (DEFB4)
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Autre désignation
- BD-4
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ID gène
- 140596
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UniProt
- Q8WTQ1
Antigène
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