CD56 anticorps

N° du produit ABIN5691114

Cet anticorps Souris Monoclonal détecte spécifiquement CD56 dans FACS, WB, ELISA, IP, IHC (p), ICC et IHC (fro). Il présente une réactivité envers Humain.

Aperçu rapide pour CD56 anticorps (ABIN5691114)

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD56 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 123A8

Détail du produit anti-CD56 anticorps

Specificité: The antibody 123A8 is directed against human NCAM/CD56 and reacts with an epitope within the membrane-proximal region of the NCAM molecule. The antibody binds to all NCAM isoforms.

Immunogène: 123A8 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with cells from a mouse immunized with a membrane preparation of a human small cell lung carcinoma

Isotype: IgG1

Information d'application

Indications d'application: The 123A8 antibody is suitable for the detection of human NCAM/CD56 by flow cytometry, immunocytochemistry, ELISA, western blotting and immunoprecipitation. The antibody is also suitable for the detection of human NCAM/CD56 by immunohistochemistry on frozen and paraffin embedded tissues. Staining of paraffin embedded tissues with 123A8 is possible following heat mediated antigen retrieval of paraffin sections using 10 mM citrate buffer, pH 6.0. Optimal antibody dilutions for the different applications should be determined by titration.

Restrictions: For Research Use only

Stockage

Buffer: Each vial contains 100 μg 1 mg/mL purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C, or in small aliquots at -20°C.

Détails sur CD56

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Autre désignation: NCAM / CD56, neural cell adhesion molecule-1

Sujet: NCAM / CD56, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by post-translational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM can be found in central and peripheral nerve cells, neuroendocrine tissues and at the surface of NK-cells. Also, NCAM is present in malignancies derived from these tissues and cells.