GFP anticorps

N° du produit ABIN5691123

Cet anticorps Lapin Polyclonal détecte spécifiquement GFP dans WB, ELISA, IF et ICC. Il présente une réactivité envers Aequorea victoria.

Aperçu rapide pour GFP anticorps (ABIN5691123)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-GFP anticorps

Specificité: The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.

Réactivité croisée (Details): Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research, embryology, and plant sciences, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols. The antibody is also suitable for immunocytochemical detection of proteins labeled with the different variants of GFP.

Purification: Immunoaffinity purified

Immunogène: The anti-GFP antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with highly purified native GFP derived from Aequorea victoria jellyfish

Isotype: Ig Fraction

Information d'application

Indications d'application: The antibody is suitable for the detection of GFP and its variants by Western blotting, ELISA and Immunocytochemistry. Optimal antibody dilutions for the different applications should be determined by titration. Recommended dilution for ELISA: 1:5,000 to 1:25,000. For immunoblotting a dilution of 1:2,000 to 1:10,000 is recommended. For immunocytochemistry the antiserum can be diluted 1:100 to 1:250.

Restrictions: For Research Use only

Stockage

Buffer: Each vial contains 25 μg 1 mg/mL immunoaffinity purified polyclonal antiserum in 10 mM TRIS buffer pH 8.0, containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C, or in small aliquots at -20°C for storage during longer periods

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: Green Fluorescent Protein

Sujet: Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research, embryology, and plant sciences, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols. The antibody is also suitable for immunocytochemical detection of proteins labeled with the different variants of GFP.