POLR2A/RPB1 anticorps (AA 1148-1384)

N° du produit ABIN5693106

Cet anticorps Lapin Polyclonal détecte spécifiquement POLR2A/RPB1 dans IHC et ELISA. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour POLR2A/RPB1 anticorps (AA 1148-1384) (ABIN5693106)

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR2A/RPB1 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA

Détail du produit anti-POLR2A/RPB1 anticorps

Épitope: AA 1148-1384

Fonction: Anti-RNA polymerase II RPB1/POLR2A Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-RNA polymerase II RPB1/POLR2A Antibody Picoband® (ABIN5693106). Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Immunogène: E. coli-derived human POLR2A recombinant protein (Position: A1148-H1384).

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: POLR2A polymerase (RNA) II (DNA ed) polypeptide A, 220 kDa". 2. Krum SA, Miranda GA, Lin C, Lane TF (December 2003). "BRCA1 associates with processive RNA polymerase II". J. Biol. Chem. 278 (52): 52012-20. 3. Scully R, Anderson SF, Chao DM, Wei W, Ye L, Young RA, Livingston DM, Parvin JD (May 1997). "BRCA1 is a component of the RNA polymerase II holoenzyme". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5605-10.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur POLR2A/RPB1

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Autre désignation: POLR2A

Sujet:

Synonyms: DNA-ed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-ed RNA polymerase II subunit A, DNA-ed RNA polymerase III largest subunit, RNA-ed RNA polymerase II subunit RPB1, POLR2A, POLR2

Background: DNA-ed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Poids moléculaire: 120 kDa

ID gène: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways