NSF anticorps (AA 620-744)

N° du produit ABIN5693126

Détail du produit anti-NSF anticorps

Antigène: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Épitope: AA 620-744

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NSF est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Marque: Picoband™

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for NSF detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogène: E. coli-derived human NSF recombinant protein (Position: N620-D744).

Information d'application

Indications d'application:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Détails sur NSF

Antigène: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Autre désignation: NSF (NSF Produits)

Synonymes: anticorps SKD2, anticorps AI316878, anticorps AU020090, anticorps AU067812, anticorps NSF, anticorps 18.m06598, anticorps nsf, anticorps si:bz18k17.1, anticorps wu:fj33g11, anticorps N-ethylmaleimide sensitive factor, anticorps T1J1.4, anticorps T1J1_4, anticorps NEM-sensitive fusion protein, anticorps N-ethylmaleimide sensitive factor, vesicle fusing ATPase, anticorps N-ethylmaleimide sensitive fusion protein, anticorps N-ethylmaleimide-sensitive factor, anticorps N-ethylmaleimide sensitive factor, anticorps N-ethylmaleimide-sensitive fusion protein, putative, anticorps N-ethylmaleimide sensitive factor L homeolog, anticorps vesicle-fusing ATPase, anticorps N-ethylmaleimide-sensitive factor a, anticorps AAA-type ATPase family protein, anticorps NSF, anticorps Nsf, anticorps nsf, anticorps BBOV_II007200, anticorps TGME49_318510, anticorps nsf.L, anticorps LOC100180685, anticorps LOC9310886, anticorps nsfa

Sujet:

Synonyms: Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, NSF

Background: N-ethylmaleimide-sensitive factor, also known as NSF, is an enzyme which in humans is encoded by the NSF gene. NSF is a homohexameric AAA ATPase involved in membrane fusion. NSF is ubiquitously found in the cytoplasm of eukaryotic cells. It is a central component of the cellular machinery in the transfer of membrane vesicles from one membrane compartment to another. During this process, SNARE proteins on two joining membranes (usually a vesicle and a target membrane such as the plasma membrane) form a tight complex. This aids fusion of the vesicle with the target membrane. It has been proposed that the role of NSF is to undo these SNARE complexes once membrane fusion has occurred, using the hydrolysis of ATP as an energy source, allowing the dissociated SNAREs to be recycled for reuse in further rounds of membrane fusion. This proposal remains controversial, however. Recent work indicates that the ATPase function of NSF does not function in recycling of vesicles but rather functions in the act of fusing vesicles with the plasma membrane.

UniProt: P46459