BRCC3 anticorps

N° du produit ABIN5700371

L’anticorps Lapin Polyclonal anti-BRCC3 a été validé pour WB, ELISA, IHC et IP. Il convient pour détecter BRCC3 dans des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour BRCC3 anticorps (ABIN5700371)

Antigène: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRCC3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit anti-BRCC3 anticorps

Fonction: BRCC3 antibody

Immunogène: BRCA1/BRCA2-containing complex, subunit 3

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

MCF7 cells were subjected to SDS PAGE followed by western blot with FNab00945(BRCC3 antibody) at dilution of 1:300

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur BRCC3

Antigène: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Autre désignation: BRCC3

Sujet:

Synonyms: Lys-63-specific deubiquitinase BRCC36|BRCA1-A complex subunit BRCC36|BRCA1/BRCA2-containing complex subunit 3|BRCA1/BRCA2-containing complex subunit 36|BRISC complex subunit BRCC36|BRCC3|BRCC36|C6.1A|CXorf53

Background: Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains(PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks(DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks(DSBs)(PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates(PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex(CSN), via the interaction of the BRISC complex with the CSN complex(PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1(PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1, deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression(PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide(LPS) via its role in IFNAR1 deubiquitination(PubMed:24075985).

Poids moléculaire: 65-70 kDa

ID gène: 79184

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus