Histone H4 anticorps (2meLys20)

N° du produit ABIN5706798

N° du produit (Fournisseur): 600-401-j02

Cet anticorps anti-Histone H4 est un anticorps Lapin Polyclonal détectant Histone H4 dans WB, IHC, IF, ChIP, DB et FM. Adapté pour Humain et C. elegans.

Aperçu rapide pour Histone H4 anticorps (2meLys20) (ABIN5706798)

Antigène: Histone H4

Reactivité: Humain, C. elegans

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Détail du produit anti-Histone H4 anticorps

Épitope: 2meLys20

N° du produit (Fournisseur): 600-401-j02

Fournisseur: Rockland

Fonction: Histone H4 K20me2 Antibody

Réactivité croisée (Details): This antibody reacts with human Histone H4.

Attributs du produit: Synonyms: rabbit anti-Histone H4 dimethyl Lys20 antibody, H4K20me2, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

Purification: Anti-Histone H4 [Dimethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H4 [Dimethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-Histone H4 [Dimethyl Lys20] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:2000

IF Microscopy Dilution: 1:50

Other: Dot Blot 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.53 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Histone H4

Antigène: Histone H4

Sujet: Background: When K20 is di-methylated on H4, the downstream effect is silencing of genes. This modification is also necessary and sufficient for 53BP1 binding, which is a prerequisite for DNA repair, a highly conserved mechanism. H4K20me2 is associated with hypoacetylation, and inactivation of certain genes. NSD1 and Suv420h1/2 trigger the dimethylation of K20, but only after SET8 catalyzes the mono-methylation of the same lysine, indicating a complex regulation of this modification that can so seriously affect replication and stability of genomic information. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.

ID gène: 121504

NCBI Accession: NP_001029249

UniProt: P62805