Histone H4 anticorps (acLys8)

N° du produit ABIN5706800

N° du produit (Fournisseur): 600-401-i99

L’anticorps Lapin Polyclonal anti-Histone H4 a été validé pour WB, IHC, IF, ChIP, DB, FM et MA. Il convient pour détecter Histone H4 dans des échantillons de Humain, Souris et C. elegans.

Aperçu rapide pour Histone H4 anticorps (acLys8) (ABIN5706800)

Antigène: Histone H4

Reactivité: Humain, Souris, C. elegans

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Détail du produit anti-Histone H4 anticorps

Épitope: acLys8

N° du produit (Fournisseur): 600-401-i99

Fournisseur: Rockland

Fonction: Histone H4 K8ac Antibody

Réactivité croisée (Details): This antibody reacts with human Histone H4.

Attributs du produit: Synonyms: rabbit anti-Histone H4 Ac Lys8 antibody, H4K8ac, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

Purification: Anti-Histone H4 [ac Lys8] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H4 [ac Lys8] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 8 of human Histone H4.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1000-1:2000

Application Note: Anti-Histone H4 [ac Lys8] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, and Immunofluorescence/Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 0.2 μg/mL

IF Microscopy Dilution: 1:1000-1:2000

Other: Dot Blot 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Histone H4

Antigène: Histone H4

Sujet: Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys5 (H4 Lys5Ac) has been linked to transcriptional activation and DNA repair. Newly assembled histones are typically acetylated on H4 at lysine 5 and 12. The enzyme histone acetyltransferase 1 (HAT1) is the primary modulator, and these marks are necessary for complete chromatin assembly. Anti-Histone H4 are ideal for researchers interested in Chromatin Research, DNA replication Transcription Translation and Splicing, Epigenetics, Chromatin Modifiers, Histones and Modified Histones, DNA Repair, and HA Epitope Tags research.

ID gène: 121504

NCBI Accession: NP_001029249

UniProt: P62805