Biotin anticorps

N° du produit ABIN5706828

N° du produit (Fournisseur): 200-301-098l

Cet anticorps anti-Biotin est un anticorps Souris Monoclonal détectant Biotin dans WB, ELISA, IHC, FACS, IF et FM. Adapté pour .

Aperçu rapide pour Biotin anticorps (ABIN5706828)

Antigène: Biotin

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Biotin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 4C7-D5

Détail du produit anti-Biotin anticorps

N° du produit (Fournisseur): 200-301-098l

Fournisseur: Rockland

Fonction: Biotin Monoclonal Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Attributs du produit: Synonyms: mouse anti-Biotin Monoclonal Antibody, Biotin Antibody, Vitamin H, Coenzyme R

Purification: Anti-Biotin Antibody was prepared from mouse ascites by Protein A chromatography sepharose beads.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).

Immunogen Type: Other

Isotype: IgG2a lambda

Information d'application

Indications d'application:

Flow Cytometry Dilution: 1-2 μg/mL

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Biotin antibody has been tested in ELISA, western, blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000

IF Microscopy Dilution: 2 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Anti-Biotin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.