EZH1 anticorps

N° du produit ABIN5706846

N° du produit (Fournisseur): 600-401-gt9

Cet anticorps anti-EZH1 est un anticorps Lapin Polyclonal détectant EZH1 dans WB et ELISA. Adapté pour Humain et Souris.

Aperçu rapide pour EZH1 anticorps (ABIN5706846)

Antigène: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EZH1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-EZH1 anticorps

N° du produit (Fournisseur): 600-401-gt9

Fournisseur: Rockland

Fonction: EZH1 Antibody

Réactivité croisée (Details): Anti-EZH1 is directed against human EZH1 at an internal position.

Attributs du produit: Synonyms: rabbit anti-EZH1 antibody, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH-1, EZH 1

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Affinity purified Anti-EZH1 antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~86 kDa in size corresponding to Ezh1 by western blotting in the appropriate cell lysate or extract. Testing using positive control NIH/3T3 nuclear extract p/n W10-001-A74.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 5.0 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur EZH1

Antigène: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Autre désignation: EZH1

Sujet: Background: Anti-Ezh1 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Anti-EZH1 Antibody detects human EZH1. Polycomb group (PcG) proteins are essential epigenetic regulators in normal tissue homeostasis and are involved in transcriptional repression. The PcG members Ezh2 and Ezh1 are important determinants of embryonic stem cell identity, and the transcript levels of these histone methyltransferases are inversely correlated during development. Recent studies have shown that EZH1 also has histone H3K27 methyltransferase activity and binds to an overlapping subset of genes. EZH1 and EZH2 have different expression patterns. EZH2 is found in actively proliferating cells, whereas EZH1 expression is higher in nonproliferative adult tissues. EZH1 partially compensates for the loss of EZH2, as shown in cells lacking only Ezh2. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. Anti-EZH1 Antibody is ideal for investigators studying pathogenesis of hematological malignancies.

ID gène: 2145

NCBI Accession: NP_001982

UniProt: Q92800