F11R anticorps (pTyr280)

N° du produit ABIN5706868

N° du produit (Fournisseur): 600-401-gn5

Cet anticorps Lapin Polyclonal détecte spécifiquement F11R dans WB, ELISA, IF et FM. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour F11R anticorps (pTyr280) (ABIN5706868)

Antigène: F11R (F11 Receptor (F11R))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp F11R est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-F11R anticorps

Épitope: pTyr280

N° du produit (Fournisseur): 600-401-gn5

Fournisseur: Rockland

Fonction: JAM A phospho Y280 Antibody

Réactivité croisée (Details): Anti-JAM A pY280 is directed against human JAM A phosphorylated at the tyrosine 280 position.

Attributs du produit: Synonyms: rabbit anti-JAM A pY280 antibody, JAM-A, Junctional adhesion molecule A, JAM-1, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, JAM1, JCAM, JAM 1, JAMA

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Affinity purified Anti-JAM A pY280 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-term and phosphorylated at the tyrosine 280 position of Human JAM A protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA, IF, and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 32.5 kDa in size corresponding to JAM A by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 5 μg/mL

IF Microscopy Dilution: User optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.95 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-F11R (ABIN5706868)

Tapia, Kralicek, Hecht: "Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells." dans: International journal of molecular sciences, Vol. 21, Issue 2, (2020) (PubMed).

Fan, Weight, Luissint, Hilgarth, Brazil, Ettel, Nusrat, Parkos: "Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation." dans: Molecular biology of the cell, Vol. 30, Issue 5, pp. 566-578, (2019) (PubMed).

Détails sur F11R

Antigène: F11R (F11 Receptor (F11R))

Autre désignation: F11R

Sujet: Background: Anti-JAM A pY280 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). JAM A pY280 Antibody detects junctional adhesion molecule A (JAM A) protein phosphorylated at the tyrosine 280 location. JAM A is involved in cell-cell contact and tight junction formation in epithelial cells. JAM A protein is a transmembrane protein expressed in epithelial and endothelial cells as well as in hematopoietic cells and is important for a variety of cellular processes including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. In addition, JAM A may be a potential therapeutic target for breast cancer.

ID gène: 50848

NCBI Accession: NP_058642

Pathways: Cell-Cell Junction Organization