Luciferase anticorps

N° du produit ABIN5706870

N° du produit (Fournisseur): 100-401-137-0100

Cet anticorps anti-Luciferase est un anticorps Lapin Polyclonal détectant Luciferase dans WB, ELISA, IHC, IP et DB. Adapté pour Vibrio fischeri. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour Luciferase anticorps (ABIN5706870)

Antigène: Luciferase

Reactivité: Vibrio fischeri

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Luciferase est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blot (DB)

Détail du produit anti-Luciferase anticorps

N° du produit (Fournisseur): 100-401-137-0100

Fournisseur: Rockland

Fonction: Luciferase Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Luciferase [Photobacterium fischerii].

Attributs du produit: Synonyms: rabbit anti-Luciferase Antibody, Alkanal monooxygenase alpha chain, Bacterial luciferase alpha chain, luxA, Alkanal monooxygenase beta chain, Bacterial luciferase beta chain, luxB

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène:

Immunogen: Luciferase [Photobacterium fischerii]

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Luciferase Antibody has been tested by dot blot and is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 75 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Références pour anticorps anti-Luciferase (ABIN5706870)

Kenny, Duval, Sammut, Steele, Pritchard, Atherton, Argent, Dimaline, Dockray, Varro: "Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).

Détails sur Luciferase

Antigène: Luciferase

Sujet: Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.

ID gène: 3280269

UniProt: P19907