Luciferase anticorps

N° du produit ABIN5706870

Détail du produit anti-Luciferase anticorps

Antigène: Luciferase

Reactivité: Vibrio fischeri

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Luciferase est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Attributs du produit:

Synonyms: rabbit anti-Luciferase Antibody, Alkanal monooxygenase alpha chain, Bacterial luciferase alpha chain, luxA, Alkanal monooxygenase beta chain, Bacterial luciferase beta chain, luxB

Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.

Gene Name: LUXB_VIBFI, luxA

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Luciferase [Photobacterium fischerii].  Cross reactivity against Luciferase from other tissues and species may occur but have not been specifically determined.

Immunogène:

Immunogen: Luciferase [Photobacterium fischerii]

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Luciferase Antibody is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 75 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 12 months

Références pour anticorps anti-Luciferase (ABIN5706870)

Kenny, Duval, Sammut, Steele, Pritchard, Atherton, Argent, Dimaline, Dockray, Varro: "Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).

Détails sur Luciferase

Antigène: Luciferase

Abstract: Luciferase Produits

Synonymes: anticorps hypothetical protein, anticorps luciferase, anticorps C25G6.3, anticorps Gbro_2186

ID gène: 3280269

UniProt: P29238, P19907