Involucrin anticorps

N° du produit ABIN5707639

Cet anticorps anti-Involucrin est un anticorps Lapin Monoclonal détectant Involucrin dans IHC (p). Adapté pour Humain.

Aperçu rapide pour Involucrin anticorps (ABIN5707639)

Antigène: Involucrin (IVL)

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Involucrin est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IVRN-2113R

Détail du produit anti-Involucrin anticorps

Purification: Purified

Pureté: Protein A affinity chromatography

Immunogène: Involucrin purified from human keratinocytes was used as the immunogen for the recombinant Involucrin antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: Titering of the recombinant Involucrin antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37oC. Enzyme digestion is more effective than Citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant Involucrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Involucrin

Antigène: Involucrin (IVL)

Autre désignation: Involucrin

Sujet: Involucrin is a highly reactive, soluble, transglutaminase substrate protein present in keratinocytes of epidermis and other stratified squamous epithelia. It first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase thus helping in the formation of an insoluble envelope beneath the plasma membrane functioning as a glutamyl donor during assembly of the cornified envelope. [Wiki]