IKZF4 anticorps (Internal Region)

N° du produit ABIN570827

L’anticorps Chèvre Polyclonal anti-IKZF4 a été validé pour WB, ELISA, IF et FACS. Il convient pour détecter IKZF4 dans des échantillons de Humain et Souris.

Aperçu rapide pour IKZF4 anticorps (Internal Region) (ABIN570827)

Antigène: IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IKZF4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-IKZF4 anticorps

Épitope: Internal Region

Fonction: IKZF4

Séquence: RPTFIDRLAN SLTKR

Specificité: This antibody is expected to recognise reported isoforms NP_071910.3, NP_001338019.1, NP_001338020.1, NP_001338021.1

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-RPTFIDRLANSLTKR, from the internal region of the protein sequence according to NP_071910.3.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line Jurkat, approx.. 60+70 kDa in nuclear lysates of cell line K562, and approx. 55 kDa in Mouse Heart lysates (calculated MW of 64.1 kDa according to Human NP_071910.3, 59.1 kDa Human NP_0

Peptide ELISA: antibody detection limit dilution 1:2000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: (5)μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur IKZF4

Antigène: IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

Autre désignation: IKZF4

Sujet: IKZF4, IKAROS family zinc finger 4 (Eos), EOS, KIAA1782, ZNFN1A4, zinc finger protein, subfamily 1A, 4, zinc finger protein, subfamily 1A, 4 (Eos), zinc finger transcription factor Eos

ID gène: 64375, 22781

NCBI Accession: NP_071910