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ITM2B anticorps (Internal Region)

Verified ITM2B Reactivité: Humain ELISA, WB, IHC, FACS, IF Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN570955
  • Antigène Voir toutes ITM2B Anticorps
    ITM2B (Integral Membrane Protein 2B (ITM2B))
    Épitope
    • 8
    • 7
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 25
    • 16
    • 2
    • 1
    Humain
    Hôte
    • 32
    • 3
    • 1
    • 1
    Chèvre
    Clonalité
    • 36
    • 1
    Polyclonal
    Conjugué
    • 17
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ITM2B est non-conjugé
    Application
    • 25
    • 20
    • 18
    • 3
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Fonction
    ITM2B
    Séquence
    HDKETYKLQR RETIK
     Réactivité croisée
    Boeuf (Vache), Humain, Souris, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1.
    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx. 30-35 kDa band observed in HepG2 cell lysates (calculated MW of 30.3 kDa according to NP_068839.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat an

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomme

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    ITM2B (Integral Membrane Protein 2B (ITM2B))
    Autre désignation
    ITM2B (ITM2B Produits)
    Synonymes
    anticorps ABRI, anticorps BRI, anticorps BRI2, anticorps BRICD2B, anticorps E25B, anticorps E3-16, anticorps FBD, anticorps imBRI2, anticorps AI256040, anticorps Bri, anticorps Bri2, anticorps Bricd2b, anticorps D14Sel6, anticorps E25BMM, anticorps itm2b, anticorps zgc:55770, anticorps zgc:85688, anticorps wu:fc05f02, anticorps MGC52745, anticorps MGC76131, anticorps ITM2B, anticorps itm2bl, anticorps wu:fb94b03, anticorps zgc:64142, anticorps integral membrane protein 2B, anticorps integral membrane protein 2Ba, anticorps integral membrane protein 2B L homeolog, anticorps integral membrane protein 2b, anticorps integral membrane protein 2Bb, anticorps ITM2B, anticorps Itm2b, anticorps itm2ba, anticorps itm2b.L, anticorps itm2b, anticorps itm2bb
    Sujet
    ITM2B, integral membrane protein 2B, ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD, BRICHOS domain containing 2B, OTTHUMP00000018394
    ID gène
    9445, 16432, 290364
    NCBI Accession
    NP_068839
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