ACPP anticorps (Internal Region)

N° du produit ABIN570986

L’anticorps Chèvre Polyclonal anti-ACPP a été validé pour WB, IHC, ELISA et IF. Il convient pour détecter ACPP dans des échantillons de Humain et Souris. Il y a 1 publication disponible.

Aperçu rapide pour ACPP anticorps (Internal Region) (ABIN570986)

Antigène: ACPP (Acid Phosphatase, Prostate (ACPP))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ACPP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-ACPP anticorps

Épitope: Internal Region

Fonction: ACPP / PAP

Séquence: YGIHKQKEKS RLQ

Specificité: This antibody is expected to recognize both isoforms (NP_001090.2, NP_001127666.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: C-YGIHKQKEKSRLQ

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5 μg/mL. This antibody has been successfully used in IHC on Mouse: Nousiainen HO et al. (2014) PMID: 2484

Western Blot: Approx 50 kDa band observed in Human Prostate lysates (calculated MW of 44.6 kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, ,Clin Chem. 1986 O

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence:: This antibody has been successfully used in IF on Mouse: Nousiainen HO et al. (2014) PMID: 24846136.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-ACPP (ABIN570986)

Nousiainen, Quintero, Myöhänen, Voikar, Mijatovic, Segerstråle, Herrala, Kulesskaya, Pulkka, Kivinummi, Abo-Ramadan, Taira, Piepponen, Rauvala, Vihko: "Mice deficient in transmembrane prostatic acid phosphatase display increased GABAergic transmission and neurological alterations." dans: PLoS ONE, Vol. 9, Issue 5, pp. e97851, (2014) (PubMed).

Détails sur ACPP

Antigène: ACPP (Acid Phosphatase, Prostate (ACPP))

Autre désignation: ACP3

Sujet: ACPP, acid phosphatase, prostate, ACP-3, ACP3, PAP, prostatic acid phosphotase

ID gène: 55, 56318, 56780

NCBI Accession: NP_001090

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process