ACAT1 anticorps (AA 253-266)

N° du produit ABIN571083

Cet anticorps anti-ACAT1 est un anticorps Chèvre Polyclonal détectant ACAT1 dans WB, ELISA, IF et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour ACAT1 anticorps (AA 253-266) (ABIN571083)

Antigène: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ACAT1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-ACAT1 anticorps

Épitope: AA 253-266

Fonction: ACAT1 (aa253-266)

Séquence: DEEYKRVDFS KVPK

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DEEYKRVDFSKVPK, from the internal region of the protein sequence according to NP_000010.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx. 40 kDa band observed in lysates of cell lines CAC02, HEK293, HepG2, MCF7, NIH3T3 and KNRK and approx. 38-40 kDa in Human, Mouse and Rat Liver lysates (calculated MW of 45.2 kDa according to Human NP_000010.1, 44.81 kDa according to Mous

Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ACAT1

Antigène: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Autre désignation: ACAT1

Sujet: ACAT1, acetyl-Coenzyme A acetyltransferase 1, ACAT, MAT, T2, THIL, acetoacetyl Coenzyme A thiolase

ID gène: 38, 110446, 25014

NCBI Accession: NP_000010