Sorting Nexin 1 anticorps (Internal Region)
Aperçu rapide pour Sorting Nexin 1 anticorps (Internal Region) (ABIN571095)
Antigène
Voir toutes Sorting Nexin 1 (SNX1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
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Épitope
- Internal Region
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Fonction
- SNX1
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Séquence
- TNSSKPQPTY EELE
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Specificité
- This antibody is expected to recognize isoform a and c (NP_003090.2, NP_690039.1.
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Réactivité croisée
- Boeuf (Vache), Chien, Humain, Souris, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogène
- Peptide with sequence C-TNSSKPQPTYEELE, from the internal region of the protein sequence according to NP_003090.2, NP_690039.1.
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry: In paraffin embedded Human Uterus shows vesicular staining in the cytoplasm of endometrial epithelium. Recommended concentration, 3-5 μg/mL.
Western Blot: Approx 75 kDa band observed in lysates of cell lines HeLa and U2OS (calculated MW of 59.1 kDa according to NP_003090.2). This molecular weight is routinely observed by other sources . Recommended concentration: 0.1-1 μg/mL. Primary incubation
Peptide ELISA: antibody detection limit dilution 1:64000.
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Commentaires
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Immunocytochemistry: Vesicular staining of SNX1 (green) in formaldehyde-fixed SH5Y5Y. Recommended concentration, 3-5μg/ml.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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