TGFBI anticorps (Internal Region)

N° du produit ABIN571098

Cet anticorps anti-TGFBI Polyclonal Chèvre (ABIN571098) détecte spécifiquement TGFBI dans WB, IHC, ELISA et IF. L’anticorps est réactif avec des échantillons de Humain et Rat.

Aperçu rapide pour TGFBI anticorps (Internal Region) (ABIN571098)

Antigène: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TGFBI est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-TGFBI anticorps

Épitope: Internal Region

Fonction: TGFBI

Séquence: QLYTDRTEKL RPE

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QLYTDRTEKLRPE, from the internal region of the protein sequence according to NP_000349.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration: 4-6 μg/mL. This antibody has been successfully used in IHC on Rat, PMID: 26407577.

Western Blot: Approx 70 kDa band observed in Human and Rat Kidney lysates, and approx 75 kDa in Human Prostate and Human Colorectal cancer lysates (calculated MW of 74.7 kDa according to Human NP_000349.1 and 74.8 kDa according to Rat NP_446254.1). Recommend

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the golgi and plasma membrane of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur TGFBI

Antigène: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Autre désignation: TGFBI

Sujet: TGFBI, transforming growth factor, beta-induced, 68 kDa, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1, RGD-containing collagen-associated protein, kerato-epithelin

Poids moléculaire: 74.7kDa

ID gène: 7045, 21810, 24511

NCBI Accession: NP_000349