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POU3F3 anticorps (Internal Region)

Cet anticorps anti-POU3F3 est un anticorps Chèvre Polyclonal détectant POU3F3 dans WB, ELISA, FACS et IF. Adapté pour Souris.
N° du produit ABIN571161

Aperçu rapide pour POU3F3 anticorps (Internal Region) (ABIN571161)

Antigène

Voir toutes POU3F3 Anticorps
POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Reactivité

  • 15
  • 3
  • 3
  • 3
  • 1
  • 1
Souris

Hôte

  • 16
  • 1
Chèvre

Clonalité

  • 17
Polyclonal

Conjugué

  • 12
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp POU3F3 est non-conjugé

Application

  • 17
  • 12
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité

Verified
  • Épitope

    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Fonction

    POU3F3 / BRN1 / OCT8

    Séquence

    HMLSHAHQWV TAL

     Réactivité croisée

    Humain, Souris, Rat

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogène

    Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.

    Isotype

    IgG
  • Indications d'application

    Western Blot: Approx 48 kDa band observed in Mouse Spinal Cord lysates and approx. 50 kDa in Mouse Brain lysates (calculated MW of 50.3 kDa according to Human NP_006227.1 and 50.2 kDa according to Mouse NP_032926.2). Recommended concentration: 1-3 μg/mL. Pri

    Peptide ELISA: antibody detection limit dilution 1:4000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Minimize freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène

    POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

    Autre désignation

    POU3F3

    Sujet

    POU3F3, POU class 3 homeobox 3, BRN1, OTF8, OTF-8, POU domain, class 3, transcription factor 3, brain-1, brain-specific homeobox/POU domain protein 1, brn-1, oct-8, octamer-binding protein 8, octamer-binding transcription factor 8

    ID gène

    5455, 18993, 192109

    NCBI Accession

    NP_006227
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