NG2 anticorps (Extracellular Domain)

N° du produit ABIN571935

Cet anticorps anti-NG2 est un anticorps Lapin Polyclonal détectant NG2 dans WB et IHC. Adapté pour Souris.

Aperçu rapide pour NG2 anticorps (Extracellular Domain) (ABIN571935)

Antigène: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NG2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-NG2 anticorps

Épitope: Extracellular Domain

Fonction: Rabbit antibody to NG2

Specificité: Specific for NG2.

Réactivité croisée: Souris

Réactivité croisée (Details): Other species not yet tested.

Purification: IgG

Immunogène: A synthetic peptide from extracellular domain of mouse NG2 conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur NG2

Antigène: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Autre désignation: NG2

Sujet: Valuable marker for several incompletely differentiated precursor cells. Function: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4 beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42 ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. Subcellular location: Apical cell membrane, Single-pass type I membrane protein, Extracellular side. Cell projection ‰ lamellipodium membrane, Single-pass type I membrane protein, Extracellular side. Tissue specificity: Expressed in microcascular pericytes and not endothelial cells.

UniProt: Q8VHY0

Pathways: Glycosaminoglycan Metabolic Process