Glutathione anticorps (DyLight 488)

N° du produit ABIN577696

Cet anticorps Souris Monoclonal détecte spécifiquement Glutathione dans FACS et IF. Il présente une réactivité envers .

Aperçu rapide pour Glutathione anticorps (DyLight 488) (ABIN577696)

Antigène: Glutathione

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Glutathione est conjugé à/à la DyLight 488

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: L4H

Détail du produit anti-Glutathione anticorps (DyLight 488)

Marque: AbX™

Attributs du produit: Designed for Immunofluorescence and FACS Analysis Supplied as 50 µg in PBS For use under Non-Reducing conditions

Purification: Purified

Immunogène: Glutathione conjugated to Keyhole Limpet Hemocyanin

Isotype: IgG2a

Information d'application

Indications d'application: 1:5-1:50 IMMuNOFLuOresCeNCe Jurkat cells, permeabilized and incubated with our DyLight® 488 labeled Glutathione monoclo- nal, A001F-100UL, clone L4H, at a 1:25 dilution. 488 Filter unfiltered
Flow analysis of Jurkat cells processed for immunofluorescence as above. Unstained cells Stained at 1x Stained at 5x

Commentaires:

Designed for Immunofluorescence and FACS Analysis Supplied as 50 μg in PBS For use under Non-Reducing conditions

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 500 μg/mL

Buffer: 500 μg/mL in Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C

Stockage commentaire: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Détails sur Glutathione

Antigène: Glutathione

Classe de substances: Chemical

Sujet: Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios. The AbX™ DyLight® 488-labeled dye is similar to Alexa 488, Cy2 or FITC. It will measure GSH-protein complexes under non-reducing conditions.