CD166 anticorps (AA 28-527) (Biotin)

N° du produit ABIN602949

Cet anticorps anti-CD166 est un anticorps Souris Monoclonal détectant CD166 dans WB et FACS. Adapté pour Humain.

Aperçu rapide pour CD166 anticorps (AA 28-527) (Biotin) (ABIN602949)

Antigène: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD166 est conjugé à/à la Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-CD166 anticorps (Biotin)

Épitope: AA 28-527

Specificité: Detects Recombinant, Human ALCAM in Western blots. No cross-reactivity seen with Recombinant, Murine ALCAM, rhBCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, r  mMadCAM or rmOCAM.

Purification: Immunoaffinity purified

Immunogène: Purified, NS0-derived, recombinant human ALCAM extracellular domain (rhALCAM, aa 28-527, Accession No. Q13740).

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhALCAM is approximately 5 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow cytometry: 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Sterile-filtered, lyophilized powder in PBS,  pH 7.2, BSA

Stock: 4 °C,-20 °C

Stockage commentaire: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Détails sur CD166

Antigène: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Autre désignation: ALCAM / CD166

Sujet: Name/Gene ID: ALCAM

Synonyms: ALCAM, CD166 antigen, MEMD, CD166

ID gène: 214

UniProt: Q13740