Histone H4 anticorps (2meLys20)
Aperçu rapide pour Histone H4 anticorps (2meLys20) (ABIN605115)
Antigène
Voir toutes Histone H4 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- 2meLys20, AA 17-28
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Marque
- IHC-plus™
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Specificité
- Recognizes methyl-Histone H4, Mr 10kD. Species cross-reactivity: Human, mouse, rat, and bovine. Broad species Cross-reactivity predicted based on conservation of sequence. Does not cross-react with yeast or Tetrahymena.
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Purification
- Protein G purified
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Immunogène
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KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 (RHR[dimeK] VLRDNIQGC) of human histone H4.
Type of Immunogen: Synthetic peptide -
Isotype
- IgG1
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Indications d'application
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Approved: IHC, IHC-P (5 μg/mL), IP, WB (0.01 - 0.1 μg/mL)
Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 0.01-0.1 μg/mL detects methyl-histone H4 in HeLa acid extracts. Minimal cross-reactivity with unmethylated recombinant Histone H4 is observed if used at these concentrations. Immunohistochemistry: stains Drosophila polytene chromosomes as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates methyl-Histone H4 from HeLa cell lysates. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS. Sourced in Ascites.
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Conseil sur la manipulation
- Aliquot to Avoid repeated freezing and thawing.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
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- Histone H4
Antigène
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