EAF2 anticorps (AA 1-170)

N° du produit ABIN6139905

Cet anticorps anti-EAF2 est un anticorps Lapin Polyclonal détectant EAF2 dans WB et IF. Adapté pour Humain.

Aperçu rapide pour EAF2 anticorps (AA 1-170) (ABIN6139905)

Antigène: EAF2 (ELL Associated Factor 2 (EAF2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EAF2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-EAF2 anticorps

Épitope: AA 1-170

Séquence: MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY LEVGEGEQVT ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL EKLSSNITVK KTRVEGSSKI QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Polyclonal Antibodies

Purification: Affinity purification

Immunogène: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human EAF2 (NP_060926.2).

Isotype: IgG

Information d'application

Indications d'application: WB,1:500 - 1:2000,IF,1:50 - 1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Détails sur EAF2

Antigène: EAF2 (ELL Associated Factor 2 (EAF2))

Autre désignation: EAF2

Sujet: Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,EAF2,BM040,TRAITS,U19,Epigenetics & Nuclear Signaling,Cancer,Tumor suppressors,Cell Biology & Developmental Biology,Apoptosis,EAF2

Poids moléculaire: 14 kDa/28 kDa

ID gène: 55840

UniProt: Q96CJ1