VWF anticorps (AA 1815-1939) (CF®647)
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- Antigène Voir toutes VWF Anticorps
- VWF (Von Willebrand Factor (VWF))
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Épitope
- AA 1815-1939
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp VWF est conjugé à/à la CF®647
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)
- Fonction
- Mouse Monoclonal anti-von-Willebrand Factor / Factor-VIII-Related Ag (VWF/1465), CF647 Conjugate
- Attributs du produit
- von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)
- Clone
- VWF-1465
- Isotype
- IgG1
- Top Product
- Discover our top product VWF Anticorps primaire
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- Indications d'application
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Immunohistology (formalin) 1-2 μg/mL
- Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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HUVEC cells or Tonsil
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- VWF (Von Willebrand Factor (VWF))
- Autre désignation
- von-Willebrand Factor / Factor-VIII-Related Ag (VWF Produits)
- Synonymes
- anticorps VWF, anticorps si:ch1073-474e24.1, anticorps F8VWF, anticorps VWD, anticorps 6820430P06Rik, anticorps AI551257, anticorps B130011O06Rik, anticorps C630030D09, anticorps von Willebrand factor, anticorps Von Willebrand factor, anticorps VWF, anticorps vwf, anticorps Vwf
- Sujet
- Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebr, Antigen 2, von Willebr, Disease (vWD)
- Poids moléculaire
- 250 kDa
- ID gène
- 7450, 440848
- UniProt
- P04275
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