VWF anticorps (AA 1815-1939) (CF®594)

N° du produit ABIN6154617

Cet anticorps Souris Monoclonal détecte spécifiquement VWF dans WB, IHC, IP, FACS et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour VWF anticorps (AA 1815-1939) (CF®594) (ABIN6154617)

Antigène: VWF (Von Willebrand Factor (VWF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VWF est conjugé à/à la CF®594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: VWF-1465

Détail du produit anti-VWF anticorps (CF®594)

Épitope: AA 1815-1939

Fonction: Mouse Monoclonal anti-von-Willebrand Factor / Factor-VIII-Related Ag (VWF/1465), CF594 Conjugate

Attributs du produit: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)

Isotype: IgG1

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HUVEC cells or Tonsil

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur VWF

Antigène: VWF (Von Willebrand Factor (VWF))

Autre désignation: von-Willebrand Factor / Factor-VIII-Related Ag

Sujet: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebr, Antigen 2, von Willebr, Disease (vWD)

Poids moléculaire: 250 kDa

ID gène: 7450, 440848

UniProt: P04275