Histone H1 anticorps (Biotin)

N° du produit ABIN6156902

Cet anticorps anti-Histone H1 est un anticorps Souris Monoclonal détectant Histone H1 dans IF, FACS et IHC (f). Adapté pour Humain, Souris et Rat.

Aperçu rapide pour Histone H1 anticorps (Biotin) (ABIN6156902)

Antigène: Histone H1

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H1 est conjugé à/à la Biotin

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HH1-957

Détail du produit anti-Histone H1 anticorps (Biotin)

Fonction: Mouse Monoclonal anti-Histone H1 (HH1/957), Biotin Conjugate

Attributs du produit: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human Histone H1 protein

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Histone H1

Antigène: Histone H1

Sujet: H1(0)|H1.1|H1.2|H1.3|H1.4|H1.5|H1A|H1F0|H1F1|H1F2|H1F3|H1F4|H1F5|H1FNT|H1FOO|H1FT|H1FV|H1FX|H1t|H1T2|H1X|HANP1|His1|HisC|HIST1|HIST1H1A|HIST1H1B|HIST1H1C|HIST1H1D|HIST1H1E|HIST1H1T|Oocyte-specific histone H1|Testicular H1 histone

Poids moléculaire: ~30 kDa

ID gène: 3005