EpCAM anticorps (Extracellular Domain) (CF®647)

N° du produit ABIN6158971

L’anticorps Souris Monoclonal anti-EpCAM a été validé pour WB, FACS, IF, ELISA et Coat. Il convient pour détecter EpCAM dans des échantillons de Humain.

Aperçu rapide pour EpCAM anticorps (Extracellular Domain) (CF®647) (ABIN6158971)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est conjugé à/à la CF®647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat)

Clone: EGP40-1798

Détail du produit anti-EpCAM anticorps (CF®647)

Épitope: AA 77-202, Extracellular Domain

Fonction: Mouse Monoclonal anti-Ep-CAM / CD326 (EGP40/1798), CF647 Conjugate

Attributs du produit: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human EpCAM protein fragment from the extracellular domain (aa77-202) (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application: ELISA 2-4 μg/mL for coating (order Ab without BSA)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HT29 cells or Ovarian carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: Ep-CAM / CD326

Poids moléculaire: 40-43 kDa

ID gène: 4072, 542050

UniProt: P16422