CEACAM1 anticorps (N-Term)

N° du produit ABIN616012

Cet anticorps anti-CEACAM1 Polyclonal Lapin (ABIN616012) détecte spécifiquement CEACAM1 dans WB, FACS et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CEACAM1 anticorps (N-Term) (ABIN616012)

Antigène: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CEACAM1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Détail du produit anti-CEACAM1 anticorps

Épitope: N-Term

Specificité: This antibody will detect recombinant Human soluble CEACAM-1. It shows cross-reactivity with several other CEACAM familiy members. There is no cross-reactivity with Human CEACAM-8.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Protein A Affinity Chromatography

Immunogène: Highly pure recombinant Human soluble CEACAM-1 (N-term)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS pH 7.4, None

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CEACAM1

Antigène: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Autre désignation: CD66a / CEACAM1

Sujet: Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1, also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa's 35-142). The three soluble forms also contain the first two C2-type Ig like domains (aa's 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57 % aa identical to human CEACAM1, in the V-type Ig-like domain, they are 58 % and 56 % aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.Synonyms: BGP, BGP1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1

ID gène: 634

NCBI Accession: NP_001703

UniProt: P13688