ESAM anticorps (AA 30-248)

N° du produit ABIN616014

L’anticorps Lapin Polyclonal anti-ESAM a été validé pour WB et IF. Il convient pour détecter ESAM dans des échantillons de Humain.

Aperçu rapide pour ESAM anticorps (AA 30-248) (ABIN616014)

Antigène: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ESAM est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-ESAM anticorps

Épitope: AA 30-248

Specificité: This antibody will detect recombinant human soluble ESAM in Western blot and native ESAM in Immunoflourescence.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Protein A affinity chromatography

Immunogène: Highly pure (>95%) recombinant human ESAM (Ile30-Ala248) derived from insect cells.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS pH 7.4 w/o preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for up to 1 month. Following reconstitution antibody can be stored at 2-8 °C for up to two weeks or (in aliquots) at -20 °C for longer.

Détails sur ESAM

Antigène: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Autre désignation: ESAM

Sujet: Endothelial cellselective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules. Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in hemophilic adhesion. In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI1. The extracellular region of human ESAM shows 90 % , 74 %, 69 % and 67 % aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell to cell junctions. Whether ESAM is restricted to a particular junctional type is not clear. ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF2. Soluble ESAM is fused to a C-terminal His-tag (6x His).Synonyms: Endothelial cell-selective adhesion molecule

ID gène: 90952

NCBI Accession: NP_620411

UniProt: Q96AP7