KRT1 anticorps

N° du produit ABIN6162000

Cet anticorps Souris Monoclonal détecte spécifiquement KRT1 dans IF, WB, FACS et IHC (f). Il présente une réactivité envers Humain, Rat, Souris, Poulet, Boeuf (Vache), Singe, Lapin et Chien.

Aperçu rapide pour KRT1 anticorps (ABIN6162000)

Antigène: KRT1 (Cytokeratin HMW (KRT1))

Reactivité: Humain, Rat, Souris, Poulet, Boeuf (Vache), Singe, Lapin, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT1 est non-conjugé

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: AE-3

Détail du produit anti-KRT1 anticorps

Fonction: Mouse Monoclonal anti-Cytokeratin, HMW (AE-3), Purified, with BSA

Attributs du produit: This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Human epidermal keratin

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Epithelial cells, Skin or Adenocarcinomas

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur KRT1

Antigène: KRT1 (Cytokeratin HMW (KRT1))

Autre désignation: Cytokeratin, HMW

Sujet: KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

Poids moléculaire: 52-67 kDa

ID gène: 51350, 654392

UniProt: Q01546