Recombinant Caldesmon HMW anticorps (CF®488A)

N° du produit ABIN6162481

L’anticorps Lapin Monoclonal anti- a été validé pour FACS, IF et IHC. Il convient pour détecter dans des échantillons de Humain et Rat.

Aperçu rapide pour Recombinant Caldesmon HMW anticorps (CF®488A) (ABIN6162481)

Antigène: Caldesmon HMW

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: CALD1-1424R

Détail du produit

Fonction: Recombinant Rabbit Monoclonal anti-Caldesmon, HMW (CALD1/1424R), CF488A Conjugate

Attributs du produit: This antibody recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human full-length CALD1 protein

Isotype: IgG kappa

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• for 10-20 min followed by cooling at RT for 20 min
• Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA
•   pH 7.5-8.5
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détail du antigène

Antigène: Caldesmon HMW

Autre désignation: Caldesmon, HMW

Sujet: CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22

Poids moléculaire: 150 kDa

ID gène: 800, 490203

UniProt: Q05682